<scp>3D</scp> Co?culturing of human neuroblastoma and human oligodendrocytes, emulating native tissue using <scp>3D</scp> porous biodegradable liquid crystal elastomers

Biomaterials cover and are an integral part of tissue engineering regenerative medicine fields. They crucial to mimic endogenous responses help eliminate high research costs ethical concerns that come with 2D systems animal models. In this study, we present a co-culturing system wherein neuroblastoma (SH-SY5Y) oligodendrocyte (MO3.13) cell lines grow within both caprolactone-based 3D elastomer liquid crystal (LCE) scaffolding. We also demonstrate LCE scaffolds promote maturation differentiation alone, in fashion as they provide suitable vitro environment study myelination. Additionally, validate these for primary growth proliferation using neurons, progenitor cells (OPCs) astrocytes. Our data suggest responsive can effectively impact cellular function, differentiation, myelination co-cultured lines, allowing the mimicry more providing support long term studies manipulate crosstalk.